14-3-3 eta, 1-246aa, Human, Recombinant

Category: Proteins
Catalog
01-2057ET
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Product Name 14-3-3 eta, 1-246aa, Human, Recombinant
Description The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3eta (eta), a subtype of the 14-3-3 family of proteins, was found in B cells, brain, cerebrospinal fluid etc. 14-3-3eta interacts with and relocalizes the A20 zinc finger protein from the insoluble to the soluble fraction, suggesting a chaperone function. Recombinant human 14-3-3eta, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
Synonyms YWHA1, YWHAH, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide, 14-3-3 eta, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide 14 3 3 protein eta, HGNC:12853, Protein AS1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide, Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide, YWHA 1.
Host E. coli
Molecular Weight 57 kDa (511 aa), confirmed by MALDI-TOF.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV LSLLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEQMQPTHP IRLGLALNFS VFYYEIQNAP EQACLLAKQA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN
Tag His-tag
Reactivity Human
Applications SDS-PAGE
Form Liquid, in Phosphate-Buffered Saline (pH 7.4)
Concentration 1 mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles.
References Sato S., et al. (2006) EMBO J 25(1):211-21 Chen J., et al. (2007), J Neurosci Res. 85(8):1724-33
Background The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3eta (eta), a subtype of the 14-3-3 family of proteins, was found in B cells, brain, cerebrospinal fluid etc. 14-3-3eta interacts with and relocalizes the A20 zinc finger protein from the insoluble to the soluble fraction, suggesting a chaperone function. Recombinant human 14-3-3eta, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
Supplier ARP

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