What is PCR?
Polymerase chain reaction (PCR) is a technique used to amplify specific DNA sequences in vitro. The PCR technique, developed in the early 80’s by Kary Mullis, is a highly efficient method of DNA amplification that has numerous applications
and has revolutionised molecular biology. The procedure relies on the use of a thermostable enzyme, DNA polymerase, which can function at temperatures more than 90oC. It also depends on the use of oligonucleotides primers that are designed
to bind to the 5’ and 3’ sequences on opposite strands of DNA, flanking specific sites of interest.
What is the process of PCR?
Polymerase chain reaction (PCR) is a technique used to amplify specific DNA sequences in vitro. The PCR technique, developed in the early 80’s by Kary Mullis, is a highly efficient method of DNA amplification that has numerous applications
and has revolutionised molecular biology. The procedure relies on the use of a thermostable enzyme, DNA polymerase, which can function at temperatures more than 90oC. It also depends on the use of oligonucleotides primers that are designed
to bind to the 5’ and 3’ sequences on opposite strands of DNA, flanking specific sites of interest.
