| Product Name | 3-NT(3-Nitrotyrosine) ELISA Kit |
|---|---|
| Description | This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 3-NT. During the reaction, 3-NT in the sample or standard competes with a fixed amount of 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 3-NT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Synonyms | 3-Nitro-L-Tyrosine, Nitrotyrosine |
| Method | Competitive ELISA |
| Detection Range | 1.56~100ng/mL |
| Sensitivity | 0.94ng/mL |
| Target | 3-NT |
| Specificity | This kit recognizes 3-NT in samples. No significant cross-reactivity or interference between 3-NT and analogues was observed. |
| Sample Types | Serum, plasma and other biological fluids |
| Reconstitution | This ELISA kit applies to the in vitro quantitative determination of 3-NT concentrations in Serum, plasma and other biological fluids. |
| Storage | An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received. |
| Supplier | Elabscience |
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