| Product Name | 6*His Monoclonal Antibody |
|---|---|
| Description | Mouse monoclonal antibody to 6xHis |
| Synonyms | anti 6*His antibody, anti 6*His antibody, anti His Tag antibody, anti 6histag antibody, anti 6XHis antibody, anti HHHHHH antibody, anti HIS antibody, anti His Tag antibody |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3G5H8 |
| Conjugate | Unconjugated |
| Immunogen | HHHHHH (6x His) synthetic peptide conjugate to KLH |
| Isotype | IgG2a |
| Target | 6*His |
| Reactivity | All |
| Applications | ELISA, WB |
| Form | Liquid |
| Diluent Buffer | Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| References | Dominguez, G. et al. Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain. Acta Neuropathol Commun 6, 25 (2018) |
| Background | A HIS tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the HIS codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound. |
| Supplier | Cusabio |
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