| Product Name | AAV2 VP1 + VP2 + VP3, recombinant proteins, set |
|---|---|
| Description | The AAV capsid consists of three capsid proteins, i.e. VP1, VP2 and VP3, which differ in their N-terminus and encapsulate the genomic ssDNA. In native virus particles, the three proteins form subunits with a ratio of 1:1:10 (VP1:VP2:VP3), in a total number of 60 subunits per capsid. This set of recombinant AAV2 VP1, VP2 and VP3 can be used to create a mixture with the precise molar ratio of 1:1:10 to compare the protein composition of the viral capsid in your sample by protein detection methods, e.g. western blot. All three recombinant AAV2 capsid proteins are available as set (Cat. No. 72001) or as individual proteins (Cat. No. 640823, 640824, 640825).Note: please find an example how to prepare western blot samples in the pipetting scheme below. Aliquots of the remaining samples can be stored at -80°C for reuse. Set content:Cat. No. 640823 AAV2 VP1, recombinant protein Cat. No. 640824 AAV2 VP2, recombinant protein Cat. No. 640825 AAV2 VP3, recombinant protein |
| Source | Escherichia coli |
| Applications | DOT, CE, SDS-PAGE, WB |
| Form | Liquid, 6 M urea in PBS |
| Concentration | 100 µg/ml (VP1: 1.19 µM, VP2: 1.45 µM, VP3: 1.61 µM) |
| Purity | > 90% (determined by SDS PAGE) |
| Storage | -80°C |
| Supplier | Progen |
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