| Product Name | ACTB CLIA kit (Human/Mouse/Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Actin Beta (ACTb). No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed. |
| Synonyms | Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed |
| Method | Sandwich |
| Detection Range | 0.14-100ng/mL |
| Sensitivity | 0.06ng/mL |
| Target | ACTB |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Beta Actin (ACTB). No significant cross-reactivity or interference between Beta Actin (ACTB) and analogues was observed. |
| Reactivity | Human, Mouse, Rat |
| Sample Types | Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 100uL Substrate Solution.Incubate 10 minutes at 37℃; 8.Read RLU value immediately. |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Chemiluminescence |
| Supplier | Cloud Clone |
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