| Product Name | Anti-26S Proteasome20S cylinder particle Monoclonal Antibody |
|---|---|
| Description | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Three transcript variants encoding two different isoforms have been found for this gene. Mouse Monoclonal Antibody to 26S Proteasome, 20S cylinder particle (p27 subunit). Polypeptide reacting: p27 subunit of 20S cylinder particles |
| Synonyms | p27, Rpn4 |
| Clonality | Monoclonal |
| Clone | P27 (26S-104) |
| Immunogen | Nuclear 26S complexes of Xenopus laevis oocytes |
| Isotype | IgG1 |
| Specificity | Recognizes the 20S subcomplex within the 26S hetero-oligomeric protein complex and the free cytosolic form of 20S cylinder particles; the epitope is resistant to formaldehyde fixation (up to 4%). |
| Reactivity | Amphibian |
| Applications | WB |
| Form | Hybridoma culture supernatant |
| Storage | 2-8C |
| References | Peters, J.M. et al. (1994) J. Biol. Chem. 269: 7708-7718Peters, J.M.: Proteasomes: protein degradation machines of the cell. TIBS, 19, September 1994 |
| Background | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Three transcript variants encoding two different isoforms have been found for this gene. |
| Supplier | ARP |
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