| Product Name | Anti-His Tag Antibody [AD1.1.10] (FITC) |
|---|---|
| Description | Mouse monoclonal [AD1.1.10] antibody to His Tag (FITC). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AD1.1.10 |
| Conjugate | FITC |
| Immunogen | PAX6 transcription factor linked to His tag. |
| Isotype | IgG1 |
| Target | His Tag |
| Specificity | This antibody recognises proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and Hwang et al. 2008) and non-mammalian (Zheng et al. 2007; Gunnarsen et al. 2010; and de Vooght et al. 2012) cell lines.In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested. |
| Reactivity | All |
| Applications | FC, IF |
| Working Dilutions | Flow Cytometry: 1:10, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide. |
| Concentration | 100 µg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. |
| Supplier | Antibodies.com |
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