| Product Name | Anti-Interferon alpha-2 (IFNA2), Monoclonal Antibody |
|---|---|
| Description | Recognizes a protein of 16-27kDa, identified as human interferon-II) (IFN-(II). Its epitope maps between aa112-148 of IFN-II) (total aa172). This monoclonal antibody is specific for IFN-(II) and does not cross-react with IFN-(I). The site recognized by this monoclonal antibody is called ðsite Ið and is responsible for the antiviral and anti-proliferative activities of IFN-(II). Epitopes of N27 and N39 monoclonal antibodyðs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-(II) after viral infections. The IFN- family consists of 24 or more genes or pseudo-genes. IFN-II) is one of the two distinct families (I and II) of human IFN-. The -interferon are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens. |
| Synonyms | Alpha 2a interferon, IFN alpha, IFN-alpha-2, IFNA, IFNA2, IFNA2B, Interferon alpha 2a, Interferon alpha 2b, Interferon alpha-2, Interferon alpha-A, LeIF2, LeIFA |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | N39 |
| Immunogen | Purified recombinant human IFN-alpha2 |
| Isotype | IgG1, kappa |
| Reactivity | Human |
| Applications | FC, IF |
| Form | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Gene Id | 3440 |
| Uniprot | P01563 |
| Storage | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
| References | Kontsek P et al. Mapping of two immunodominant structures on human interferon alpha 2c and their role in binding to cells. Mol Immunol 1991, 28:1289-1297 Kontsek P et al. Peptide-mapping of three neutralizing epitopes into predicted biologically active sites of human interferon-alpha 2. Immunol Lett 1993, 35(3):281-284 Pestka S et al. Interferons and their actions. Annu Rev Biochem 1987, 56:727-777 Sen GC et al.The interferon system. A bird's eye view of its biochemistry. J Biol Chem 1992, 267(8):5017-5020Capon DJ et al. Two distinct families of human and bovine interferon-alpha genes are coordinately expressed and encode functional polypeptides. Mol Cell Biol 1985, 5(4):768-779Kurane I et al. Induction of interferon alpha from human lymphocytes by autologous, dengue virus-infected monocytes. J Exp Med 1987, 166(4):999-1010Lepe-Zuniga JL et al. Production of interferon-alpha induced by dsRNA in human peripheral blood mononuclear cell cultures: role of priming by dsRNA-induced interferons-gamma and -beta. J Interferon Res 1989, 9(4):445-456Aman MJ et al. Interferon-alpha stimulates production of interleukin-10 in activated CD4+ T cells and monocytes. Blood 1996, 87(11):4731-473 |
| Supplier | ARP |
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