| Product Name | Biotinylated human FcRn (FCGRT-B2M) heterodimer protein |
|---|---|
| Description | The sequence of the extracellular domain of human FCGRT (Ala 24-Ser 297) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag. This was co-transfected with the sequence of human B2M (Ile 21-Met 119) and the resulting FcRn heterodimer was purified. The full protein sequences can be downloaded from the product webpage. |
| Synonyms | FcRn, FCGRT & B2M, Neonatal Fc receptor |
| Source | Human |
| Conjugate | Biotin |
| Molecular Weight | The recombinant FcRn heterodimer including tags consists of 412 amino acids and has a theoretical mass of 46570 Da. The heterodimer consists of human FCGRT (313 amino acids; 34839 Da) and human B2M (99 amino acids; 11731 Da). |
| Expression System | Human embryonic kidney (HEK) 293 cells. |
| Form | Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. |
| Purity | >95% monomer purity as determined by SDS-PAGE and SEC-HPLC. |
| Gene Id | P55899 / P61769 |
| Storage | Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial. |
| Notes | Conjugation notes: Biotinylated. Biotin to protein ratio is confirmed as 0.7-1.0 by the HABA assay. Product has been site-specifically biotinylated using the AVI tag technology, where the lysine residue within the tag is enzymatically labeled with biotin. .. Allotype: Not applicable. Reconstitution: To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water. |
| Supplier | Gamma Proteins |
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