Chicken Rudimental Bovine Serum albumin (BSA) check-up ELISA Kit
| Product Name | Chicken Rudimental Bovine Serum albumin (BSA) check-up ELISA Kit |
|---|---|
| Description | Chicken Rudimental Bovine Serum albumin (BSA) check-up ELISA Kit has high sensitivity and excellent specificity for detection of Chicken BSA. No significant cross-reactivity or interference between Chicken BSA and analogues was observed. BSA is a serum albumin protein that has numerous biochemical applications. It is also used as a nutrient in cell and microbial culture. BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. This protein does not affect other enzymes that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. BSA is used because of its stability, its lack of effect in many biochemical reactions, and its low cost since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry. The nickname "Fraction V" refers to albumin being the fifth fraction of the original Edwin Cohn purification methodology that made use of differential solubility characteristics of plasma proteins.adopted for production of BSA. This Chicken Rudimental Bovine Serum albumin (BSA) check-up ELISA Kit employs a two-site sandwich ELISA to quantitate BSA in samples. An antibody specific for BSA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBSA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BSA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BSA bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | BSA |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Chicken |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | BSA is a serum albumin protein that has numerous biochemical applications. It is also used as a nutrient in cell and microbial culture. BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. This protein does not affect other enzymes that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. BSA is used because of its stability, its lack of effect in many biochemical reactions, and its low cost since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry. The nickname "Fraction V" refers to albumin being the fifth fraction of the original Edwin Cohn purification methodology that made use of differential solubility characteristics of plasma proteins.adopted for production of BSA. |
| Supplier | Abbkine |
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