Bovine 28S ribosomal protein S36, mitochondrial (MRPS36) ELISA Kit
| Product Name | Bovine 28S ribosomal protein S36, mitochondrial (MRPS36) ELISA Kit |
|---|---|
| Description | Bovine 28S ribosomal protein S36, mitochondrial (MRPS36) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MRPS36. No significant cross-reactivity or interference between Bovine MRPS36 and analogues was observed. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. The mitochondrial ribosome (mitoribosome) consists of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS36 encodes a 28S subunit protein. Pseudogenes corresponding to this gene are found on chromosomes 3p, 4q, 8p, 11q, 12q, and 20p. This Bovine 28S ribosomal protein S36, mitochondrial (MRPS36) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS36 in samples. An antibody specific for MRPS36 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRPS36 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS36 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS36 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MRPS36, DC47, MGC22896, MRP-S36, |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 613835 |
| Storage | 2-8°C |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. The mitochondrial ribosome (mitoribosome) consists of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS36 encodes a 28S subunit protein. Pseudogenes corresponding to this gene are found on chromosomes 3p, 4q, 8p, 11q, 12q, and 20p. |
| Supplier | Abbkine |
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