| Product Name | Bovine Lipopolysaccharide binding protein (LBP) ELISA Kit |
|---|---|
| Description | Bovine Lipopolysaccharide binding protein (LBP) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine LBP. No significant cross-reactivity or interference between Bovine LBP and analogues was observed. LPS-binding protein (LBP) is serum factor known to regulate the endotoxin-induced cellular immune response. Sepsis is a morbid condition induced by a toxin, the introduction or accumulation of which is most commonly caused by infection or trauma. Sepsis-inducing toxins have been found associated with pathogenic bacteria, viruses, plants and venoms.Among the well described bacterial toxins are the endotoxins or lipopolysaccharides (LPS) of the gram-negative bacteria. Upon introduction of LPS into the blood it binds to lipopolysaccharide binding protein (LBP). LBP recognizes the lipid A region of LPS and forms high affinity complexes with both rough and smooth form LPS. During the acute phase, LBP is synthesized by hepatocytes, and reaches very high concentrations in serum. This Bovine Lipopolysaccharide binding protein (LBP) ELISA Kit employs a two-site sandwich ELISA to quantitate LBP in samples. An antibody specific for LBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LBP bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | LBP, MGC22233, LPS-binding protein, lipopolysaccharide-binding protein |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 512242 |
| Storage | 2-8°C |
| Background | LPS-binding protein (LBP) is serum factor known to regulate the endotoxin-induced cellular immune response. Sepsis is a morbid condition induced by a toxin, the introduction or accumulation of which is most commonly caused by infection or trauma. Sepsis-inducing toxins have been found associated with pathogenic bacteria, viruses, plants and venoms.Among the well described bacterial toxins are the endotoxins or lipopolysaccharides (LPS) of the gram-negative bacteria. Upon introduction of LPS into the blood it binds to lipopolysaccharide binding protein (LBP). LBP recognizes the lipid A region of LPS and forms high affinity complexes with both rough and smooth form LPS. During the acute phase, LBP is synthesized by hepatocytes, and reaches very high concentrations in serum. |
| Supplier | Abbkine |
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