| Product Name | EEF1B2, 1-225aa, Human |
|---|---|
| Description | Elongation factor 1-beta, also known as EEF1B2, is a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. EEF1B2 and EEF1D stimulate the exchange of GDP bound to EEF1A to GTP. Recombinant human EEF1B2 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Elongation factor 1-beta, EF-1-beta, EEF1B, EEF1B1, EF1B |
| Host | E. coli |
| Molecular Weight | 24.1 kDa (221aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGFGDLKSPA GLQVLNDYLA DKSYIEGYVP SQADVAVFEA VSSPPPADLC HALRWYNHIK SYEKEKASLP GVKKALGKYG PADVEDTTGS GATDSKDDDD IDLFGSDDEE ESEEAKRLRE ERLAQYESKK AKKPALVAKS SILLDVKPWD DETDMAKLEE CVRSIQADGL VWGSSKLVPV GYGIKKLQIQ CVVEDDKVGT DMLEEQITAF EDYVQSMDVA AFNKILEHHH HHH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.2M NaCl, 1mM DTT, 0.1mM PMSF |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Byun HO., et al. (2009) Cancer Res, 69(11):4638-47. Sang Lee J., et al. (2002) Biochem Biophys Res Commun. 291(1):158-64. |
| Background | Elongation factor 1-beta, also known as EEF1B2, is a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. EEF1B2 and EEF1D stimulate the exchange of GDP bound to EEF1A to GTP. Recombinant human EEF1B2 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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