| Product Name | Goat anti-PALS / MPP5 Antibody |
|---|---|
| Synonyms | MPP5; PALS1; FLJ12615; membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5); stardust (Drosophila); MAGUK p55 subfamily member 5; membrane protein, palmitoylated 5; stardust |
| Host | Goat |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | NKASPPFPLISNAQ-C |
| Molecular Weight | 77.3; 73.4 |
| Target | PALS / MPP5 |
| Amino Acid Sequence | NKASPPFPLISNAQ |
| Reactivity | Bovine, Canine, Human, Porcine |
| Applications | ELISA, FC, IF, IHC |
| Working Dilutions | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Form | Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin |
| Diluent Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reconstitution | WB: Preliminary testing showed a band at approx 75kDa in Human Cerebellum lysate after 1µg/ml antibody staining (calculated MW of 73.4kDa according to NP_001243479.1). Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Small Intestine and Kidney. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. |
| Gene Id | Human GeneIDs: 64398 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| References | Kamberov E, Makarova O, Roh M, Liu A, Karnak D, Straight S, Margolis B. , Molecular cloning and characterization of Pals, proteins associated with mLin-7., J Biol Chem. 2000 Apr 14;275(15):11425-31., PMID: 10753959 |
| Supplier | Everest Biotech |
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