Goat anti-XBP1 / TREB5 Antibody

Category: Antibodies
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EB08557
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Product Name Goat anti-XBP1 / TREB5 Antibody
Synonyms XBP1; TREB5; X-box binding protein 1 ; XBP2; OTTHUMP00000028682; X-box-binding protein-1
Host Goat
Clonality Polyclonal
Conjugate Unconjugated
Immunogen C-NHSWEDTFANELFPQ
Molecular Weight 40.1; 28.7
Target XBP1 / TREB5
Amino Acid Sequence NHSWEDTFANELFPQ
Reactivity Canine, Human, Mouse, Rat
Applications ELISA, IF, IHC, WB
Working Dilutions Peptide ELISA: antibody detection limit dilution 1:4000.
Form Liquid, in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Diluent Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Reconstitution WB: Approx 38kDa band observed in Nuclear lysates of cell line HeLa (calculated MW of 40.1kDa according to NP_001073007.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour.at room temperature.. IHC: Paraffin embedded Human Breast. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Expression of the protein seen in the endoplasmic reticulum, cytoplasm and nucleus of HeLa and MCF7 cells. Recommended concentration: 10µg/ml.
Gene Id Human GeneIDs: 7494. Non-Human GeneIDs: 22433 (mouse);
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
References Yokouchi M, Hiramatsu N, Hayakawa K, Okamura M, Du S, Kasai A, Takano Y, Shitamura A, Shimada T, Yao J, Kitamura M., Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response., J Biol Chem. 2007 Dec 17; [Epub ahead of print], PMID: 18086661
Supplier Everest Biotech

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