| Product Name | HMGB1 ELISA kit (Mouse) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMG1). No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMG1) and analogues was observed. |
| Synonyms | HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein |
| Method | Sandwich |
| Detection Range | 46.88-3,000pg/mL |
| Sensitivity | 18.29pg/mL |
| Target | HMGB1 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMGB1). No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMGB1) and analogues was observed. |
| Reactivity | Mouse |
| Sample Types | Serum, Plasma And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P63158 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice;The Inhibitory Effect of Lidocaine on the Release of High Mobility Group Box 1 in Lipopolysaccharide-Stimulated Macrophages;Simvastatin suppresses vascular inflammation and atherosclerosis in ApoE-/- mice by downregulating the HMGB1-RAGE axis;Ascorbic acid reduces HMGB1 secretion in lipopolysaccharide-activated RAW 264.7 cells and improves survival rate in septic mice by activation of Nrf2/HO-1 signals;HMGB1 is an early and critical mediator in an animal model of uveitis induced by IRBP-specific T cells;Diabetes-Induced Oxidative Stress in Endothelial Progenitor Cells May Be Sustained by a Positive Feedback Loop Involving High Mobility Group Box-1;Retinoic acid inhibits tissue factor and HMGB1 via modulation of AMPK activity in TNF-α activated endothelial cells and LPS-injected mice;Glycyrrhizin reduces HMGB1 secretion in lipopolysaccharide-activated RAW 264.7 cells and endotoxemic mice by p38/Nrf2-dependent induction of HO-1;HMGB1 expression patterns during the progression of experimental autoimmune encephalomyelitis;HMGB1 release triggered by the interaction of live retinal cells and uveitogenic T cells is Fas/FasL activation-dependent;Interleukin-33 is released in spinal cord and suppresses experimental autoimmune encephalomyelitis in mice;Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1/RAGE/NF-ΚB pathway.;Oxidative Stress in Metabolic Disorders: Pathogenesis, Prevention, and Therapeutics;Zoledronic acid-encapsulating self-assembling nanoparticles and doxorubicin: a combinatorial approach to overcome simultaneously chemoresistance and immunoresistance in breast tumors;Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathway;Reduced HMGB 1-Mediated Pathway and Oxidative Stress in Resveratrol-Treated Diabetic Mice: A Possible Mechanism of Cardioprotection of Resveratrol in …;High Mobility Group Box1 Protein Is Involved in Endoplasmic Reticulum Stress Induced by Clostridium difficile Toxin A;HMGB1 exacerbates experimental mouse colitis by enhancing innate lymphoid cells 3 inflammatory responses via promoted IL-23 production;Glycyrrhizin ameliorates experimental colitis through attenuating interleukin-17-producing T cell responses via regulating antigen-presenting cells.;Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats.;Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced …;Mineral particles stimulate innate immunity through neutrophil extracellular traps containing HMGB1;Macrophage Inflammatory Protein-2 in High Mobility Group Box 1 Secretion of Macrophage Cells Exposed to Lipopolysaccharide.;AMP-activated protein kinase agonist N 6-(3-hydroxyphenyl) adenosine protects against fulminant hepatitis by suppressing inflammation and apoptosis;Glycyrrhizin Protects Mice against Experimental Autoimmune Encephalomyelitis by Inhibiting HMGB1 Expression and Neuronal HMGB1 Release;Ursolic Acid Ameliorates Inflammation in Cerebral Ischemia and Reperfusion Injury Possibly via High Mobility Group Box 1/Toll-Like Receptor 4/NFκB …;Luteolin activates ERK1/2- and Ca-dependent HO-1 induction that reduces LPS-induced HMGB1, iNOS/NO, and COX-2 expression in RAW264.7 cells and …;Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced mice;Sirt1 S-nitrosylation induces acetylation of HMGB1 in LPS-activated RAW264. 7 cells and endotoxemic mice;Butyrate suppresses abnormal proliferation in colonic epithelial cells under diabetic state by targeting HMGB1;Delivery of 5'-Triphosphate RNA with Endosomolytic Nanoparticles Potently Activates RIG-I to Improve Cancer Immunotherapy;Apoptosis resistance in fibroblasts precedes progressive scarring in pulmonary fibrosis and is partially mediated by Toll-like receptor 4 activation;Magnetic hyperthermia using self-controlled heating elements consisting of Fe-Al milling alloy induces cancer cell apoptosis while preserving skeletal muscle;Suppression of high-mobility group box 1 ameliorates xerostomia in a sjogren syndrome-triggered mouse model;Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis;Inhibition of calpain alleviates coxsackievirus B3-induced myocarditis through suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and …;A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem …;A non-lethal murine flame burn model leads to a transient reduction in host defenses and enhanced susceptibility to lethal Pseudomonas aeruginosa infection;Inhaled [D-Ala2]-Dynorphin 1-6 Prevents Hyperacetylation and Release of High Mobility Group Box 1 in a Mouse Model of Acute Lung Injury;Calycosin attenuates severe acute pancreatitis-associated acute lung injury by curtailing high mobility group box 1-induced inflammation; |
| Supplier | Cloud Clone |
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