| Product Name | HTGL, Human, ELISA Kit |
|---|---|
| Description | HTGL (hepatic triacylglycerol lipase / hepatic triglyceride lipase / (HL) hepaticlipase ), a lipolytic enzyme, is a secreted glycoprotein. HTGL plays a major role in lipoprotein metabolism as a lipolytic enzyme that hydrolyzes triglycerides (TGs) and phospholipids in chylomicron remnants, intermediate-density lipoproteins (IDLs), and high-density lipoproteins (HDLs). It has been reported that the persons with HTGL deficiency present with hypercholesterolemia or hypertriglyceridemia and accumulate b-very-low-density lipoproteins (VLDLs), chylomicron remnants, IDLs,TG-rich low-density lipoproteins (LDLs) and HDLs. HTGL is synthesized by hepatocytes and bound to heparin sulfate proteoglycans at the surface of liver sinusoidal capillaries. Measurements of HTGL in plasma havebeen routinely carried out after intravenous injection of heparin (post heparin plasma) as well as lipoprotein lipase (LPL). Because of the high binding affinity of heparin for the lipases, the enzyme bound to endothelial surfaces is rapidly released by heparin from capillary beds into plasma. This kit is a solid phase sandwich ELISA using 2 kinds of highly specific antibodies. Tetra Methyl Benzidine (TMB) is used as a coloring agent (Chromogen). The strength of coloring is proportional to the quantities of Human HTGL. |
| Reactivity | Human |
| Background |
HTGL (hepatic triacylglycerol lipase / hepatic triglyceride lipase / (HL) hepaticlipase ), a lipolytic enzyme, is a secreted glycoprotein. HTGL plays a major role in lipoprotein metabolism as a lipolytic enzyme that hydrolyzes triglycerides (TGs) and phospholipids in chylomicron remnants, intermediate-density lipoproteins (IDLs), and high-density lipoproteins (HDLs). It has been reported that the persons with HTGL deficiency present with hypercholesterolemia or hypertriglyceridemia and accumulate b-very-low-density lipoproteins (VLDLs), chylomicron remnants, IDLs,TG-rich low-density lipoproteins (LDLs) and HDLs. HTGL is synthesized by hepatocytes and bound to heparin sulfate proteoglycans at the surface of liver sinusoidal capillaries. Measurements of HTGL in plasma havebeen routinely carried out after intravenous injection of heparin (post heparin plasma) as well as lipoprotein lipase (LPL). Because of the high binding affinity of heparin for the lipases, the enzyme bound to endothelial surfaces is rapidly released by heparin from capillary beds into plasma. |
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