| Product Name | Human C-Peptide ELISA Kit ELISA Kit |
|---|---|
| Synonyms | H3R2me2a, H3t, H3.4, H3/g, H3FT, |
| Host | Rabbit |
| Method | Competitive |
| Detection Range | 0.312 - 20 ng/ml |
| Sensitivity | 0.148 ng/ml |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding R2 of human histone H3 |
| Isotype | IgG |
| Molecular Weight | 16kDa |
| Target | C Peptide ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Working Dilutions |
WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 CHIPseq 1:20 - 1:100 |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Form | Liquid, in PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Reconstitution | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample. |
| Purity | Affinity purification |
| Gene Id | 8290 |
| Uniprot | Q16695 |
| Storage | Store at -20C. Avoid freeze/thaw cycles. |
| Supplier | Antibodies.com |
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