| Product Name | Human Cytochrome b5 reductase 4 (CYB5R4) ELISA Kit |
|---|---|
| Description | Human Cytochrome b5 reductase 4 (CYB5R4) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYB5R4. No significant cross-reactivity or interference between Human CYB5R4 and analogues was observed. Cytochrome b5 reductase 4 is an enzyme that in humans is encoded by the CYB5R4 gene. NCB5OR is a flavohemoprotein that contains functional domains found in both cytochrome b5 (CYB5; MIM 250790) and CYB5 reductase (DIA1; MIM 250800). This Human Cytochrome b5 reductase 4 (CYB5R4) ELISA Kit employs a two-site sandwich ELISA to quantitate CYB5R4 in samples. An antibody specific for CYB5R4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYB5R4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYB5R4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYB5R4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | CYB5R4, NCB5OR, RP4-676J13.1, dJ676J13.1, NADPH cytochrome B5 oxidoreductase, cytochrome b-type NAD(P)H oxidoreductase, flavohemoprotein b5+b5R |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 51167 |
| Storage | 2-8°C |
| Background | Cytochrome b5 reductase 4 is an enzyme that in humans is encoded by the CYB5R4 gene. NCB5OR is a flavohemoprotein that contains functional domains found in both cytochrome b5 (CYB5; MIM 250790) and CYB5 reductase (DIA1; MIM 250800). |
| Supplier | Abbkine |
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