Human Fc gamma RIIIa / CD16a (176V) protein

Category: Proteins
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HUGR3AV-U
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Product Name Human Fc gamma RIIIa / CD16a (176V) protein
Description The sequence of the extracellular domain of human CD16a (Gly 17-Gln 208) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag. The full protein sequence can be downloaded from the product webpage.
Synonyms CD16A, FCGR3A, FCGRIIIA, FCR3A, FCRIIIA, IGFR3A, IGFRIIIA
Source Human
Conjugate Unconjugated
Molecular Weight The recombinant human CD16a (176V) including tag consists of 231 amino acids and has a theoretical mass of 26251 Da.
Expression System Human embryonic kidney (HEK) 293 cells.
Form Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added.
Purity >95% monomer purity as determined by SDS-PAGE and SEC-HPLC.
Gene Id P08637
Storage Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.
Notes Conjugation notes: Unconjugated (no label). The protein contains an AVI tag but this has not been biotinlyated in this product... Allotype: 176V. Reconstitution: To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.
Supplier Gamma Proteins

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