| Product Name | Human FcRn (FCGRT-B2M) heterodimer protein |
|---|---|
| Description | The sequence of the extracellular domain of human FCGRT (Ala 24-Ser 297) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag. This was co-transfected with the sequence of human B2M (Ile 21-Met 119) and the resulting FcRn heterodimer was purified. The full protein sequences can be downloaded from the product webpage. |
| Synonyms | FcRn, FCGRT & B2M, Neonatal Fc receptor |
| Source | Human |
| Conjugate | Unconjugated |
| Molecular Weight | The recombinant FcRn heterodimer including tags consists of 412 amino acids and has a theoretical mass of 46570 Da. The heterodimer consists of human FCGRT (313 amino acids; 34839 Da) and human B2M (99 amino acids; 11731 Da). |
| Expression System | Human embryonic kidney (HEK) 293 cells. |
| Form | Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. |
| Purity | >95% monomer purity as determined by SDS-PAGE and SEC-HPLC. |
| Gene Id | P55899 / P61769 |
| Storage | Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial. |
| Notes | Conjugation notes: Unconjugated (no label). The protein contains an AVI tag but this has not been biotinlyated in this product... Allotype: Not applicable. Reconstitution: To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water. |
| Supplier | Gamma Proteins |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
