| Product Name | Human Gamma-glutamylcysteine synthetase (γ-GCS) ELISA Kit |
|---|---|
| Description | Human Gamma-glutamylcysteine synthetase (γ-GCS) ELISA Kit has high sensitivity and excellent specificity for detection of Human γ-GCS. No significant cross-reactivity or interference between Human γ-GCS and analogues was observed. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.It catalyses the ATP-dependent condensation of cysteine and glutamate to form the dipeptide gamma-glutamylcysteine. The peptide bond in this peptide product is between the carboxylate group of the glutamate and the amino group of the cysteine.The product of the GCL-mediated condensation reaction of L-glutamate and L-cysteine is gamma-glutamylcysteine, which is readily condensed with glycine, by glutathione synthetase, to form glutathione. This Human Gamma-glutamylcysteine synthetase (γ-GCS) ELISA Kit employs a two-site sandwich ELISA to quantitate γ-GCS in samples. An antibody specific for γ-GCS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyγ-GCS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for γ-GCS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of γ-GCS bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | γ-GCS, glutamate-cysteine ligase, gamma-glutamylcysteine synthetase |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Human |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.It catalyses the ATP-dependent condensation of cysteine and glutamate to form the dipeptide gamma-glutamylcysteine. The peptide bond in this peptide product is between the carboxylate group of the glutamate and the amino group of the cysteine.The product of the GCL-mediated condensation reaction of L-glutamate and L-cysteine is gamma-glutamylcysteine, which is readily condensed with glycine, by glutathione synthetase, to form glutathione. |
| Supplier | Abbkine |
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