| Product Name | IDO ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Indoleamine-2,3-Dioxygenase (IDO). No significant cross-reactivity or interference between Indoleamine-2,3-Dioxygenase (IDO) and analogues was observed. |
| Synonyms | CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase |
| Method | Sandwich |
| Detection Range | 0.312-20ng/mL |
| Sensitivity | 0.127ng/mL |
| Target | IDO |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Indoleamine-2,3-Dioxygenase (IDO). No significant cross-reactivity or interference between Indoleamine-2,3-Dioxygenase (IDO) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P14902 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjects;Potential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCT;Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell;CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic Mechanisms;Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinoma;Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans;The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered;Chronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytes;Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic properties;Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenase;Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells;Richard Schäfer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Kuçi, 4;Altered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes?¡;Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4;Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive …; |
| Supplier | Cloud Clone |
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