| Product Name | Mouse Delta (24)-sterol reductase (DHCR24) ELISA Kit |
|---|---|
| Description | Mouse Delta (24)-sterol reductase (DHCR24) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DHCR24. No significant cross-reactivity or interference between Mouse DHCR24 and analogues was observed. DHCR24 encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in DHCR24 have been associated with desmosterolosis. Also, reduced expression of DHCR24 occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. This Mouse Delta (24)-sterol reductase (DHCR24) ELISA Kit employs a two-site sandwich ELISA to quantitate DHCR24 in samples. An antibody specific for DHCR24 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHCR24 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHCR24 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHCR24 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | DHCR24, KIAA0018, Nbla03646, SELADIN1, seladin-1, 3 beta-hydroxysterol delta 24-reductase, diminuto/dwarf1 homolog, putative protein product of Nbla03646, selective AD indicator 1 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Mouse |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 74754 |
| Storage | 2-8°C |
| Background | DHCR24 encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in DHCR24 have been associated with desmosterolosis. Also, reduced expression of DHCR24 occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. |
| Supplier | Abbkine |
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