| Product Name | OC ELISA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Osteocalcin (OC). No significant cross-reactivity or interference between Osteocalcin (OC) and analogues was observed. |
| Synonyms | BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein |
| Method | Sandwich |
| Detection Range | 15.6-1,000pg/mL |
| Sensitivity | 6.3pg/mL |
| Target | OC |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Osteocalcin (OC). No significant cross-reactivity or interference between Osteocalcin (OC) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P04640 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Biochemical study of the influence of ad type biophytomodulators in healing of bone defects in rats.;Oxytocin promotes bone formation during the alveolar healing process in old acyclic female rats;Exendin-4 Increases Bone Mineral Density in Type 2 Diabetic OLETF Rats Potentially Through the Down-Regulation of SOST/Sclerostin in Osteocytes;Serum changes induced by intramedullar experimental administration of bisphosphonates;Effect of the “protein diet” and bone tissue.;Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions;Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats;Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation;Transplantation of mesenchymal stem cells, recombinant human BMP-2,and their combination in accelerating the union after osteotomy and increasing, the mechanical strength of extracorporeally irradiated femoral autograft in rat models;The influence of bexarotene, a selective agonist of the retinoid receptor X (RXR), and tazarotene, a selective agonist of the retinoid acid receptor (RAR), on bone metabolism in rats;Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway;Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathway;Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats;The effects of strength training and raloxifene on bone health in aging ovariectomized rats;Effect of Sodium Fluoride on Bone Biomechanical and Histomorphometric Parameters and on Insulin Signalingand Insulin Sensitivity in Ovariectomized Rats.;Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats;The controlled release of simvastatin from TiO2 nanotubes to promote osteoblast differentiation and inhibit osteoclast resorption;Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats.;Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway;Effects of long-term administration of pantoprazole on bone mineral density in young male rats.;Effects of fluoride on insulin signaling and bone metabolism in ovariectomized rats.;Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats;Oral administration of kaempferol inhibits bone loss in rat model of ovariectomy-induced osteopenia;A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/ò-catenin Signaling Pathways in Ovariectomized Rats;BMP-2 gene transfection of bone marrow stromal cells to induce osteoblastic differentiation in a rat calvarial defect model;Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats;Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model;Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway;Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats;In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells;The decline of whole-body glucose metabolism in ovariectomized rats;Mollugin attenuates glucocorticoid-induced osteoporosis in rats via Akt/P13K pathway;Influence of physical training on markers of bone turnover, mechanical properties, morphological alterations, density and mineral contents in the femur of rats exposed …;Methylbenzoxime as a therapeutic agent for glucocorticoid-induced osteoporosis in rats.;Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and …;Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar Rats;Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signals;;Bioinspired polydopamine/graphene oxide/collagen nanofilms as a controlled release carrier of bioactive substances;Effects of icariin on the fracture healing in young and old rats and its mechanism;Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway;Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing; |
| Supplier | Cloud Clone |
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