| Product Name | Protein Disulfide Isomerase (PDI), human |
|---|---|
| Description | Recombinant human Protein Disulfide Isomerase (rHuPDI). rHuPDI is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. rHuPDI is a single, non-glycosylated, polypeptide chain containing 503 amino acids and having a molecular mass of 62.4kDa.The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Ala. rHuPDI is contained N-terminal 6xHis-tag. |
| Synonyms | Protein kinase C alpha type, EC 2.7.11.13, PKC-alpha, PKC-A, PRKCA, AAG6, PKCA, PRKACA, MGC129900, MGC129901 |
| Molecular Weight | 62.4 kDa |
| Biological Activity | Isomerase Activity: 0.5 µmol active RNase A min-1 µmol PDI-1. According to the re-activation of reduced and denatured RNase A (Lyles, M. M. &Gilbert, H. F. (1991) Biochemistry 30, 613-619).Reductase Acticity: 0.001 650nm/ min-2. By measuring the turbidity increase at 650 nm due to insulin reduction (Holmgren, A. (1979) J. Biol. Chem. 254, 96279632). The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time (Mart?´nez-Galisteo, E., Padilla, C. A., Garcia-Alfonso, C., Lo´pez-Barea, J., and Barcena, J. A. (1993) Biochimie (Paris) 75, 803809) |
| Form | Purified, in PBS, pH 7 |
| Concentration | 1 mg/ml protein |
| Purity | > 95% by SDS-PAGE |
| Storage | 2-8C for immediate use, or at -2°C (aliquot) |
| Supplier | ARP |
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