| Product Name | PtX Anti-His Tag (C-term) (3D5) Recombinant Antibody (HRP) |
|---|---|
| Description | Horseradish peroxidase (HRP) conjugated recombinant monoclonal antibody against C-terminal polyhistidine tagged proteins. This product is produced in Nicotiana benthamiana plants and is suitable for Western Blot and ELISA applications. |
| Synonyms | polyhis tag antibody, anti-his, 6xhis tag antibody, histidine antibody, his tag, his-tagged protein |
| Host | Plant |
| Source | Human |
| Clonality | Monoclonal |
| Sensitivity | Refer to ELISA dose response graph in Datasheet for ELISA sensitivity. |
| Clone | 3D5 |
| Isotype | IgG1 |
| Molecular Weight | ± 150 kDa |
| Target | Polyhistidine tag/ purified his-tagged Fab fragment at the carboxy-terminal |
| Expression System | N. Benthamiana plants |
| Applications | ELISA, WB |
| Working Dilutions | Suggested dilution is 1: 500 - 1: 5 000 for WB and 1: 1 000 - 1: 150 000 for ELISA. Optimal dilutions/concentrations should be determined by the user. |
| Form | Liquid |
| Diluent Buffer | 0.1 M Phosphate Buffered Saline (PBS), 25% glycerol preservative, pH = 7.4 |
| Concentration | 0.5 mg/ml |
| Purity | ≥ 95 % as determined by SDS-PAGE. |
| Uniprot | NA |
| Storage | -20°C Freezer |
| Notes | Purified using Protein A affinity chromatography |
| Supplier | Cape Biologix Technologies |
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