Rat Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) ELISA Kit
| Product Name | Rat Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) ELISA Kit |
|---|---|
| Description | Rat Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DMTF1. No significant cross-reactivity or interference between Rat DMTF1 and analogues was observed. DMTF1 encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer. This Rat Cyclin-D-binding Myb-like transcription factor 1 (DMTF1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMTF1 in samples. An antibody specific for DMTF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMTF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMTF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMTF1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | DMTF1, DMP1, DMTF, FLJ25188, FLJ41265, FLJ76054, hDMP1, OTTHUMP00000205131, cyclin D-binding Myb-like protein 1 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Rat |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 114485 |
| Storage | 2-8°C |
| Background | DMTF1 encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer. |
| Supplier | Abbkine |
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