| Product Name | Rat Defensin Beta 1 ELISA Kit |
|---|---|
| Synonyms | H3K27me2, HIST1H3J, H3/j , H3FJ , Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h , Histone H3/I, Histone H3/j , Histone H3/k, Histone H3/l, HIST3H3, |
| Host | Rabbit |
| Method | Sandwich |
| Detection Range | 0.781 - 50 ng/ml |
| Sensitivity | 0.27 ng/ml |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
| Isotype | IgG |
| Molecular Weight | 15kDa |
| Target | Defensin Beta 1 |
| Reactivity | Rat |
| Applications | ELISA |
| Working Dilutions |
WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 CHIPseq 1:20 - 1:100 |
| Sample Types | Serum, plasma, tissue homogenates and other biological fluids. |
| Form | Liquid, in PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Reconstitution | The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| Purity | Affinity purification |
| Gene Id | 8290 |
| Uniprot | Q16695 |
| Storage | Store at -20C. Avoid freeze/thaw cycles. |
| Supplier | Antibodies.com |
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