Rat sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
| Product Name | Rat sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit |
|---|---|
| Description | This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TCCC5b-9 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TCCC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TCCC5b-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TCCC5b-9. The concentration of Rat TCCC5b-9 in samples can be calculated by comparing the OD of the samples to the standard curve. |
| Synonyms | MAC, Membrane Attack Complex |
| Method | Sandwich ELISA |
| Detection Range | 0.16~10ng/mL |
| Sensitivity | 0.1ng/mL |
| Target | sC5b-9 |
| Specificity | This kit recognizes Rat sC5b-9 in samples. No significant cross-reactivity or interference between Rat sC5b-9 and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, plasma and other biological fluids |
| Reconstitution | This ELISA kit applies to the in vitro quantitative determination of Rat sC5b-9 concentrations in Serum, plasma and other biological fluids. |
| Storage | An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received. |
| Supplier | Elabscience |
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