Recombinant Human DNA dC->dU-editing enzyme APOBEC-3A (APOBEC3A)
| Product Name | Recombinant Human DNA dC->dU-editing enzyme APOBEC-3A (APOBEC3A) |
|---|---|
| Synonyms | Phorbolin-1 |
| Source | Yeast |
| Molecular Weight | 24.5 kDa |
| Amino Acid Sequence | MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTSVKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWGCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWDTFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN |
| Protein Length | Full Length |
| Tag | C-terminal 6xHis-tagged |
| Working Dilutions | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Form | Liquid or Lyophilized powder |
| Diluent Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Uniprot | P31941 |
| Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week. |
| References | "Psoriasis upregulated phorbolin-1 shares structural but not functional similarity to the mRNA-editing protein apobec-1."Madsen P.P., Anant S., Rasmussen H.H., Gromov P., Vorum H., Dumanski J.P., Tommerup N., Collins J.E., Wright C.L., Dunham I., Macginnitie A.J., Davidson N.O., Celis J.E.J. Invest. Dermatol. 113:162-169(1999) |
| Supplier | Cusabio |
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